Nuance Multispectral Tissue Imaging System

Basic Information
Perkin Elmer
Name/Model #: 
Nuance 3.0.2
Slide Scanner and quantitative biomarker analysis (self-serve)
Location Information
Contact Information
Contact Name: 
Karla Esbona, Ph.D
Phone number: 
Advanced Information
Features & Accessories: 

Flexible bandwidth multispectral microscopy. The Nuance® FX multiplex biomarker imaging system is specifically tailored to meet the majority of all brightfield and fluorescence microscopy imaging applications. The system utilizes an optimized, high-throughput tunable filter distinctly matched to the bandwidths of common molecular markers. This solid-state filter is electronically switchable to maximize either sensitivity or throughput, enabling the Nuance FX to provide either chromogenic or fluorescence-based imaging. Nuance acquisition software incorporates easy to use tools for building custom spectral libraries, as well as powerful unmixing and analysis algorithms allowing spectral characterization and quantitation for each of the multi-labeled components in an image. inForm advanced image analysis software is also an available option for rapid analysis of targeted tissues and batch processing of user-specified regions across multiple images.



Company Link to Description 

Brightfield (BF) Fluorescence(Fl)

BF, Fl

Slide scanning


Single slide capacity

Multiplexing analysis

Up to 14 channels

Auto-Fl. removal


Spectral Library


Analysis Software

Nuance and inForm

Data Format

Ordinal & Continuous

Suggested Applications:

Biomarker quantification
Co-localization & quantification

Per-tissue analysis (epith. vs. stroma)

Subcellular quantification

Per-cell data

Tissue structure (vessel, glomerulus, etc) counting