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Nipkow-disc based Confocal Microscope with Deconvolution
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This is a service instrument for use by any group that needs it. The microscope can be operated by BBPIC staff, or investigators may be trained for independent use. Features: A mercury arc lamp is used for fluorescence microscopy instead of lasers. This gives a wide choice of fluorophores, and is not limited to 2 or 3 specific excitation lines, as with lasers. Specimens may be examined by transmitted brightfield illumination, transmitted-light differential interference contrast (DIC), epi-fluorescence, or in confocal mode using a spinning Nipkow disc. This confocal mode requires a reasonably bright signal, but gives the potential of studying living cells and samples that are sensitive to the phototoxicity and photobleaching of laser based systems. Images collected by any of these methods may then be deconvolved using the microscope's software. The software and hardware are fully integrated so that the imaging program acquires the information needed to do the deconvolution along with the image. Image recording is done with a cooled CCD monochrome digital camera. Image files may be saved in the Zeiss format or in JPG, TIF, or other formats. Training: Instrument scheduling: for online schedule and sign-up click here.
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